Active regions (ARs) are a candidate source of the slow solar wind (SW), the origins of which are a topic of ongoing research. We present a case study that examines the processes by which SW is modulated in the presence of an AR in the vicinity of the SW source. We compare properties of SW associated with a coronal hole (CH)–quiet Sun boundary to SW associated with the same CH but one Carrington rotation later, when this region bordered the newly emerged NOAA AR 12532. Differences found in a range of in situ parameters are compared between these rotations in the context of source region mapping and remote sensing observations. Marked changes exist in the structure and composition of the SW, which we attribute to the influence of the AR on SW production from the CH boundary. These unique observations suggest that the features that emerge in the AR-associated wind are consistent with an increased occurrence of interchange reconnection during SW production, compared with the initial quiet Sun case.