Effects of Non-Inhibitory Serpin Maspin on the Actin Cytoskeleton: A Quantitative Image Modelling Approach

Mohammad Al-Mamun, Lorna Ravenhill, Worawut Srisukkham, Alamgir Hossain, Charles Fall, Vincent Ellis, Rosemary Bass

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
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Recent developments in quantitative image analysis allow us to interrogate confocal microscopic images to answer biological questions. Clumped and layered cell nuclei and cytoplasm in confocal microscopic images challenges the ability to identify subcellular compartments. To date, there is no perfect image analysis method to identify cellular cytoskeletal changes from confocal microscopic images. Here we present a multi-disciplinary study where an image analysis model was developed to allow quantitative measurements of changes in the cytoskeleton of cells with different maspin exposure. Maspin, a non-inhibitory serpin influences cell migration, adhesion, invasion, proliferation and apoptosis in ways that are consistent with its identification as a tumor metastasis suppressor. Using different cell types we tested the hypothesis that the reduction of cell migration by maspin would be reflected in the architecture of the actin cytoskeleton. A hybrid marker controlled watershed segmentation technique was used to segment the nuclei, cytoplasm and ruffling regions prior to measuring cytoskeletal changes. This was informed by immunohistochemical staining of cells transfected stably or transiently with maspin proteins, or with added bioactive peptides or protein. Image analysis results showed that the effects of maspin were mirrored by effects on cell architecture, in a way that could be described quantitatively.
Original languageEnglish
Pages (from-to)394-409
JournalMicroscopy and Microanalysis
Issue number02
Publication statusPublished - Apr 2016


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